What we can expect T999 do
Gain Muscle
According to a paper by Kazuyoshi Shigehara, Yuki Kato and others, there is a positive correlation between total testosterone levels and muscle growth [1]. T999 can significantly increase testosterone levels, so its usefulness in helping muscle growth is self-evident.
In addition, a fruit fly experiment showed that compared with the blank control group, the chest protein content of aged fruit flies increased by 16.4% after feeding T999 (0.05g/L). This experiment indirectly shows that T999 has a positive correlation with muscle growth.
Fig. 5 Effects of T999 on muscle mass in male fruit flies (*P<0.05, **P<0.01 VS control group)
Experimental institution: Hunan Key Laboratory of Small Molecule Drug Discovery and Translation for Chronic Disease Diagnosis and Treatment.
Experimental Conditions:
1. Preparation of Culture Media
● Add T999 to the ordinary culture medium to create drug-containing media at concentrations of 0.02 g/L and 0.05 g/L.
● For the blank control group, prepare a 20% sucrose solution.
● For the positive control group, select young fruit flies and add a 20% sucrose solution.
2. Culturing
Culture the fruit flies in the respective media for a specified period.
3. Sample Collection
After the culture period, collect the thoraces of fruit flies from all groups.
4. Protein Extraction
Add SDS lysis buffer to the collected thoraces and grind to extract the protein.
5. Centrifugation
Centrifuge the mixture at a specific temperature to separate the supernatant from the cellular debris.
6. Protein Concentration Determination
Measure the protein concentration in the supernatant using the BCA method.
This protocol outlines the steps for evaluating the effects of T999 on protein content in fruit flies, contributing to insights into its potential impact on muscle growth.
[1] SHIGEHARA K, KATO Y, IZUMI K, et al. Relationship between Testosterone and Sarcopenia in Older-Adult Men: A Narrative Review [J]. J Clin Med, 2022, 11(20).
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